The C3H10T1/2 cells model with different BMP2 gene variants were constructed and subjected to BLU-9931 mechanical stretch. The results suggested the expression of BMP2 transfected by wild-type or mutant-type expression vectors was significantly higher than the un-transfected group, but there was no statistical difference between them in the static condition. These results were consistent with that of ligament tissues from OPLL patients with different genotypes. Meantime, in the presence of mechanical stress, the C3H10T1/2 cells transfected by wild-type or mutant-type expression vectors aggregated more intensely and gradually manifested to the osteoblastic differentiation and the expression of BMP2 protein transfected by pcDNA3.1-BMP2 were significantly higher than the corresponding static groups. The existing research results suggested the BMP2 gene variant of rs2273073 increased sensibility to the mechanical stress which could affect the cellular morphology, promote the osteogenic differentiation. Ossification of the posterior longitudinal ligament is a significantly critical pathology that can eventually cause serious myelopathy. OPLL was first reported by Key and later by Oppenheimer. However, this disease attracted our attentions as a cause of myelopathy only after the report by Tsukimoto in 1960. The involvement of multiple etiologic factors in the development of OPLL has been suggested, including genetic factors, metabolic abnormalities, dietary habits, and some local factors. There are numerous clinical studies about the progression of OPLL under mechanical stress. OPLL cells have been transformed into cells that are highly sensitive to mechanical stress, which may induce the progression of OPLL. OPLL cells can detect and transduce mechanical FRAX597 stretch into biochemical signals that can modulate locomotion. Meanwhile, research suggested stretch-dependent Ca2+ influx through SA channels was essential in the stretch-dependent cell orientation and elongation which contributed to the progression of ossification.In present study, we observed the similar effects of cyclic stretch on morphology changes of the C3H10T1/2 cells and our experimental results are supported by those mentioned above.