As an example, the widely-used GISTIC method for determining frequent aberrations uses as its test statistic, at each locus, the number of samples in which a gain is present multiplied by the mean amplitude of the gain. However, both the count and the mean amplitude depend on earlier choices in the pipeline. These changes in membrane structure, as well as dehydration-induced HbC crystallization and increased internal viscosity, are believed to play some role in the mild anemia that homozygous CC individuals experience as a result of accelerated erythrocyte turnover. Therefore, unlike Rab5, Rab21 seems not to be necessary for macropinosome formation. It is possible that Rab21 may play a unique role in macropinocytosis, although Rab21 shares some characteristics with Rab5. On the other hand, proteins involved the later stages of cancer development might be important for disease maintenance/aggression and are potential prognostic factors and/or drug targets. While it is intuitive to select these conserved residues across the subfamily, by a visual analysis of a multiple alignment, however, one tends to miss out the residues which may have a lower conservation but still are functionally important. Epithelial cells are characterized by an apical junctional complex, comprising tight junctions, adherens junctions and desmosomes. TJ and AJ are of critical importance in the development and physiology of vertebrate epithelial tissues. TJ control the barrier function of epithelia and maintain cell polarity, and AJ regulate cell-cell adhesion and morphogenesis. The molecular architecture of TJ and AJ has been largely clarified in recent years : junctions comprise transmembrane proteins that are linked to cytoskeletal filaments through a cytoplasmic plaque, that contains scaffolding, adaptor and signaling proteins. As CaMdr1p is an antiporter and belongs to DHA1 family. We then scaled the CRE across the two families with the conservation in DHA1 which helped us to enlist the alignment positions on the basis of high CRE as well as high conservation across the entire DHA1 family. This enabled us to identify residues conserved exclusively in DHA1 and those which were differentially conserved among the two families. Our analysis revealed that all the mutant variants except P139A displayed decreased resistance to the tested drugs though at varying degree and specificity. To directly study the interaction between the amino-terminus and the carboxyl terminus of aSyn in neurons we overexpressed doubly-tagged Myc-aSyn-V5 in both mouse and rat primary neuronal cultures. Neurons were transfected at 5-7 days in vitro using VE-821 Lipofectamine 2000 and transfection efficiencies in the order of 5% were achieved. Immunostaining using antibodies against Myc and V5 confirmed that both epitope tags were expressed and completely colocalized at the subcellular level.