All reactions other than ligand syntheses were performed under an inert atmosphere of argon using either glovebox or Schlenk line techniques. Toluene and hexane were distilled from sodium and phosphorus pentoxide respectively. This mechanism provides insight into how these pyridyl triazole ligands can also act as corrosion inhibitors for aluminum, and further studies are underway to determine their feasibility. The family of cysteine rich proteinscomprises three closely related members, CRP1, CRP2, and CRP3. CRP3 is also known as muscle LIM proteinand has been postulated as a muscle-specific protein. It is involved in the myogenesis and cytoskeletal organization of myocytes. Accordingly, MLP deficiency leads to myocardial hypertrophy followed by cardiomyopathy and heart failure. In myocytes, MLP is localized in the cytoplasm, where it is involved in cytoskeleton modulation. In addition, MLP has been reported to translocate to the nucleus to modulate gene expression during myogenesis and as part of a biomechanical stress response. A Danshensu functional role of MLP in other tissues such as the central nervous system, such as the retina has not yet been described. Amacrine cellsare a heterogeneous group of retinal interneurons and Ganoderic-acid-G synaptically interact with bipolar cells, retinal ganglion cellsand other AC. Mammalian AC have been classified into more than 26 morphologic subtypesthat are distinguishable by specific molecular markers, morphology, size and their neurotransmitters. Cholinergic AC produce the neurotransmitter acetylcholineallowing their specific identification by antibodies against choline acetyltransferase. In rodents, ChAT-immunoreactive AC are detected from embryonic day 17 and produce acetylcholine lifelong. AC are either conventionally located in the inner nuclear layerat the border with the inner plexiform layeror displaced to the ganglion cell layer. The cells of the displaced subpopulation are stimulated by light and therefore termed ONcells. Their dendrites are restricted to the ON sublamina of the IPL, whereas the AC located in the INL are excited in the absence of light. These AC accumulate their dendrites in the OFF-sublamina. As a consequence of this strict stratification pattern, two distinct ChAT-immunoreactive dendritic layers of cholinergic AC are visible in the IPL. Moreover, cholinergic AC induce spontaneous waves of action potentials in the developing retina, which reportedly facilitates the formation of visual circuits between retinal neurons. This process is apparently driven by the release of acetylcholine. In mammals, the morphology of cholinergic AC proceeds development until the animals open their eyes between postnatal days 13�C15. Concurrently, AC form synapses with direction-selective RGCs during the first two postnatal weeks to establish functional circuits. Islet1, NeuroD or Math3 are reportedly among the few so far identified factors that promote genesis and differentiation of AC, while cell adhesion and guidance molecules such as semaphorins participate in the specific laminar stratification of AC. The current study reports that MLP is expressed in the cytoplasm of cholinergic AC during the late embryonic and the postnatal maturation stage, thereby demonstrating that MLP is also markedly expressed in other tissue than muscle and that MLP is a specific marker for postnatal cholinergic AC with a potential role in AC maturation. MLP is a well-characterized protein, mainly expressed in heart tissue.