The graphical view of expression of these fasciclin-like arabinogalactan genes in the seed

These data aid in determining perturbations in the physiological development of the plant cell wall which affects agronomically important traits and is a source of bioenergy. The graphical representations of the expression levels of the other differentially expressed genes shown in Tables 3 and 4 at all three different stages of seed development is presented in Figures S6 and S7, respectively, showing that all have the highest differential expression at the mid- developmental stages of 100�C 200 mg seed weight. Included is the pattern for several other proline and glycine-rich proteins and that of the xylem serine proteinase that is overexpressed in the defective seed coats. In many of these genes, those that are highly expressed in the 50�C100 mg seed coats and then decline show a pattern similar to PRP1 in that they are overexpressed in the defective seed coats. Likewise, those that are overexpressed in the standard line are similar to PRP2 in that they generally have higher RPKM expression levels later in development. Cobicistat Figure 3 shows that 21% of the cell wall related genes were annotated as fasciclin related. The graphical view of expression of these fasciclin-like arabinogalactan genes in the seed coats of both standard and defective seed coat isolines at three different stages of seed development is presented in Figure S8. All of the genes Azlocillin sodium salt showed expression patterns with significant differential expression at 100�C200mg seed weight stage and their transcript levels were higher in the defective as compared to the standard seed coats, as was the case with PRP1. Thus, all 11 of these genes were overexpressed in the defective seed coat. Figure S9 graphs the developmental profiles of the remaining 25 cell wall genes overexpressed in the defective isolines and Figure S10 shows the remaining 3 cell wall genes that were overexpressed in the standard seed coats. It is clear from the 68 graphs of the gene expression patterns shown in Figures S6�CS10 that the 100�C200 mg weight range shows the highest differential expression and that many of the genes can be classified as either declining in expression or increasing in expression during seed coat development as is the case for the PRP1 and PRP2 patterns. In many cases, the net pattern mutation appears to increase expression of many of the cell wall related genes in the 100�C200 mg weight range as for PRP1.

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