This study was conducted to examine the specific-hypothesis that resemblance between the C-termini of the two T. cruzi ribosomal proteins TcP0/TcP2b and prior suspected causative insults for EMF explains the common-ality of gross pathology. Initially designed to comprise an initial exploratory In Silico phase exploiting comparative sequence alignments and subsequent In Situ hybridization proof of PD325901 concept phase, the herein presented non-specific results of the exploratory phase made it difficult to conduct parallel confirma-tory In Situ inquiry due to a wide spectrum of test candidates and limited resources. Specifically, contrarily to prior data pointing to an architectural conservation of ribosomal P protein-structure across some life domains, no sequence similarity was found between the acidic termini of T.cruzi ribosomal P proteins TcP0/ TcP2b and sequences of all searched plant, microbial and viral databases by initial NCBI microbial BLAST-P at default. Repeat BLAST at SIB, however, revealed that both C-termini of T. cruzi ribosomal P protein TcP0 and TcP2b exhibit homology to acidic termini of respective eukaryotic proteins. Further, the C-termini of TcP0 and TcP2b are noted to possess characteristic amino acid composition that confer unto them acidity and negative charge. Overall, we provide evidence to suggest that cross reactivity of antibodies against C-terminal sequences of several animal,Perifosine plant and protozoal ribosomal P proteins with heart tissue may mediate EMF in a similar manner as C-termini of T. cruzi do for Chaga’s disease. It is, never the less, still possible that the mechanisms of molecular mimicry between the suspected EMF-insults and myocardial tissue are mediated via different myocardial antigens altogether-thereby, making the specified protein-portions in our study not the likely cause of EMF. Our findings offer the first ever evidence to support the postulate that cross reactivity of antibodies against C-terminal sequences of ribosomal P proteins from several animals, plant and protozoal with heart tissue may mediate EMF in a similar manner as C-termini of T. cruzi do for Chaga’s disease.